Intern - Research and Early Development - Molecular Oncology
United States, California, South San Francisco
Start Date: Summer 2018Length of Assignment: 12 weeks The mission of the Molecular Oncology department is to bring lasting benefit and cures to cancer patients through innovative scientific discoveries and medicines.
We will transform cancer care by taking a science-driven approach to drug discovery and development to achieve enduring clinical benefit.
Guided by a deep understanding of the unique molecular features associated with each patient's disease, we will identify and understand pathways that drive cancer and establish the therapeutic potential of individual targets.
By combining laboratory and clinical investigation, we will design strategies and medicines that target tumor cells, drug resistance, and the tumor microenvironment to achieve dramatic, lasting, benefit for cancer patients.
Position 1This intern will work with a postdoctoral fellow to investigate the impact of a kinase on the interaction between CD8 T cells and Antigen Presenting Cells (APC). He or she will use FACS analysis and various imaging techniques to investigate the above question. Position 2
Generate and test mammalian cells edited by CRISPR technology.
Perform various assays including western blotting, qPCR, ELISA, FACS to determine changes in gene expression.
Optimize culture conditions for primary cells and conduct in vitro phagocytosis assay.
Position 3Metastases are the principal cause of death in patients with colorectal cancer (CRC). In order to develop effective methods for prevention and targeting of metastatic disease, it is of crucial importance to better understand the cellular and molecular mechanisms regulating the metastatic cascade. The project will consist in evaluating the role of specific regulatory regions and signaling pathways in mediating the metastatic ability of colon cancer cells. First, the intern will have to design, clone and validate CRISPR-based tools (CRISPR KO or CRISPR i) to specifically and efficiently target genes and regulatory regions found in our in vivo screening and thought to be playing a key role in colon cancer cells metastatic ability. Also, the intern will be involved in setting up a drug screening in order to assess the effect of different compounds on colon cancer cells in vitro.
In hiring new employees, we look for people who are also inspired by our mission and who would fit in well with the collaborative, rigorous and entrepreneurial spirit of the company culture. Because we know that employees are critical to our success in bringing novel medicines to patients, we are dedicated to remaining a great place to work and to providing employees with programs, services and benefits that allow them to bring the best to the business and to their personal lives. Position 1
Background in Molecular Biology, Cell Biology, Genetics, Biochemistry
Previous experience with cell culture and molecular biology techniques (cloning, digestion, DNA preparation...) is highly desired
Previous experience with flow cytometry analysis and/or imaging techniques (confocal microscopy...) is preferred
Applicants with good understanding of concepts in cancer biology/genomics/epigenetics will be prioritized
A member of the Roche Group, Genentech has been at the forefront of the biotechnology industry for more than 40 years, using human genetic information to develop novel medicines for serious and life-threatening diseases. Genentech has multiple therapies on the market for cancer & other serious illnesses. Please take this opportunity to learn about Genentech where we believe that our employees are our most important asset & are dedicated to remaining a great place to work.
Roche is an equal opportunity employer and strictly prohibits unlawful discrimination based upon an individual's race, color, religion, gender, sexual orientation, gender identity/expression, national origin/ancestry, age, mental/physical disability, medical condition, marital status, veteran status, or any other characteristic protected by law.
If you have a disability and need an accommodation in connection with the on-line application process, please email us at .
PAY TRANSPARENCY NONDISCRIMINATION PROVISION The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor's legal duty to furnish information. 41 CFR 60-1.35(c)
Location United States, California, South San Francisco
Function Technical Development
Subfunction Internship / Trainee Technical Development
Job level Entry Level
Job type Internship / Student
Division Roche Pharmaceuticals
Posted since 2018/01/12
Posted until 2018/02/03
Roche is an Equal Opportunity Employer & prohibits unlawful discrimination based on race, color, religion, gender, sexual orientation, gender identity/expression, national origin/ancestry, age, disability, marital & veteran status.